In the accompanying sequence listing: SEQ ID NO: 1 shows the nucleic acid sequence of the SARS-CoV genome.
SEQ ID NO: 2 shows the amino acid sequence of the SARS-CoV polyprotein 1a (encoded by какие симптомы при коронавирусе у взрослых nucleic acid 265 to nucleic acid 13,398 of SEQ ID NO: 1). SEQ ID NO: 3 shows the amino acid sequence of the какие симптомы при коронавирусе у взрослых SARS-CoV polyprotein 1b (encoded by nucleic acid 13,398 to 21,482 of SEQ ID NO: 1).
SEQ ID NO: 4 shows the amino acid sequence of the SARS-CoV S protein (encoded by nucleic acid 21,492 to 25,256 of SEQ ID NO: 1). SEQ ID NO: 5 shows the amino acid sequence of the SARS-CoV X1 protein (encoded by nucleic acid 25,268 to 26,089 of SEQ ID NO: 1). SEQ ID NO: 6 shows the amino acid sequence of the SARS-CoV X2 protein (encoded by nucleic acid 25,689 to 26,150 of SEQ ID NO: 1).
SEQ ID NO: 7 shows the amino acid sequence of the SARS-CoV E protein (encoded by nucleic acid 26,117 to 26,344 of SEQ ID NO: 1). SEQ ID какие симптомы при коронавирусе у взрослых NO: 8 shows the amino acid sequence of the SARS-CoV M protein (encoded by nucleic acid 26,398 to 27,060 of SEQ ID NO: 1).
SEQ ID NO: 9 shows the amino acid sequence of какие симптомы при коронавирусе у взрослых the SARS-CoV X3 protein (encoded by nucleic acid 27,074 to 27,какие симптомы при коронавирусе у взрослых 262 of SEQ ID NO: 1). SEQ ID NO: 10 shows the какие симптомы при коронавирусе у взрослых amino acid sequence of the SARS-CoV X4 protein (encoded by nucleic acid 27,273 to 27,638 of SEQ ID NO: 1). SEQ ID NO: 11 shows the amino acid sequence of the SARS-CoV X5 protein (encoded by nucleic acid 27,864 to 28,115 of SEQ ID NO: 1). SEQ ID NO: 12 shows the amino acid sequence of the SARS-CoV N protein (encoded by nucleic acid 28,120 to 29,385 of SEQ ID NO: 1).
SEQ ID NOs: 13–15 show the nucleic acid sequence of several SARS-CoV-specific oligonucleotide primers. SEQ ID NOs: какие симптомы при коронавирусе у взрослых 16–33 show the nucleic acid sequence of several oligonucleotide primers/probes used for какие симптомы при коронавирусе у взрослых real-time reverse transcription-polymerase chain reaction (RT-PCR) SARS-CoV assays. SEQ ID NOs: 34–35 какие симптомы при коронавирусе у взрослых show the nucleic acid sequence of two degenerate primers designed to anneal to sites encoding conserved coronavirus amino acid motifs. SEQ ID NOs: 36–38 show the nucleic acid sequence of several oligonucleotide primers/probe used as controls in real-time RT-PCR assays. Abbreviations M: coronavirus membrane protein N: coronavirus nucleoprotein какие симптомы при коронавирусе у взрослых ORF: open reading frame PCR polymerase chain reaction RACE: 5? rapid amplification какие симптомы при коронавирусе у взрослых of cDNA ends RT-PCR: reverse transcription-polymerase chain reaction S: coronavirus spike protein SARS: severe acute respiratory syndrome SARS-CoV: severe acute respiratory syndrome-associated coronavirus какие симптомы при коронавирусе у взрослых TRS: transcriptional regulatory sequence II. Terms Unless otherwise noted, technical terms are used according to conventional usage.
Definitions of common terms in molecular biology may be found in Benjamin Lewin, Genes VII , published by Oxford University какие симптомы при коронавирусе у взрослых Press, 2000 (ISBN 019879276X); Kendrew et al. (eds.), The Encyclopedia of Molecular Biology , published by Blackwell Publishers, 1994 (ISBN 0632021829); and Robert A. Meyers (ed.), Molecular Biology and Biotechnology: a Comprehensive Desk Reference , published by Wiley, какие симптомы при коронавирусе у взрослых John & Sons, Inc., 1995 (ISBN 0471186341); and other similar references. As used herein, the singular terms “a,” “an,” and “the” include plural referents unless context clearly indicates otherwise. Similarly, the word “or” is intended to include “and” unless the context clearly indicates otherwise. Also, as used herein, the term “comprises” means “includes.” Hence “comprising A or B” means including A, B, or A and B. It is further to be understood that all nucleotide sizes or amino acid sizes, and all molecular weight or molecular mass values, given for nucleic acids or polypeptides are approximate, and are provided for description. Although methods and materials similar or equivalent to those described herein can be used in the practice or testing of the present invention, suitable methods and materials are described below. All какие симптомы при коронавирусе у взрослых publications, patent applications, patents, and other references mentioned herein are incorporated by reference in their entirety. In case of conflict, the present specification, including explanations of terms, will control. In addition, the materials, methods, and examples are illustrative only and not intended to be limiting. In order to facilitate review of the various embodiments of this disclosure, the following explanations of specific terms are provided: Adjuvant: A substance that non-specifically enhances какие симптомы при коронавирусе у взрослых the immune response to an antigen. Development of vaccine adjuvants for use in humans is reviewed in Singh et al. 17:1075–1081, 1999), which discloses that, at the time of its publication, aluminum salts and the MF59 microemulsion are the only vaccine adjuvants approved for human use. Amplification: Amplification of a nucleic acid molecule (e.g., a DNA or RNA molecule) refers to use of a laboratory technique that increases the какие симптомы при коронавирусе у взрослых number of copies of a nucleic acid molecule in a sample. An example of amplification is the polymerase chain reaction (PCR), in which a sample is contacted with a pair of oligonucleotide primers under conditions that какие симптомы при коронавирусе у взрослых allow for the hybridization of the primers to a nucleic acid template in the sample. The primers are extended under suitable conditions, dissociated from the template, re-annealed, extended, and dissociated to amplify the number of copies of the nucleic acid. The product of amplification can be characterized by какие симптомы при коронавирусе у взрослых such techniques as electrophoresis, restriction endonuclease cleavage patterns, oligonucleotide hybridization or ligation, and/or nucleic acid sequencing. Other examples of amplification methods include strand displacement amplification, as disclosed in U.S. 5,744,311; transcription-free isothermal amplification, as disclosed in U.S. 6,033,881; repair chain reaction amplification, as disclosed in WO 90/01069; ligase chain reaction amplification, as disclosed in EP-A-320,308; gap filling ligase chain reaction amplification, as disclosed in U.S. 5,427,930; and NASBA™ RNA transcription-free amplification, as disclosed in U.S. An amplification method can be modified, including for example by additional steps or coupling the amplification with another protocol.
Animal: Living multi-cellular vertebrate organisms, a category that includes, for example, mammals and birds. The term mammal includes both human and non-human mammals. Similarly, the term “subject” includes both human and veterinary subjects, for example, humans, non-human primates, dogs, cats, horses, and cows. Antibody: A protein (or protein complex) that includes one or more polypeptides substantially encoded by immunoglobulin genes or fragments of immunoglobulin genes. The recognized immunoglobulin genes include the kappa, lambda, alpha, gamma, delta, epsilon, and mu constant region genes, as well as the myriad immunoglobulin variable region genes. Light chains are classified as either kappa or lambda.
Heavy chains are classified as gamma, mu, alpha, delta, or epsilon, which in turn define the immunoglobulin classes, IgG, IgM, IgA, IgD and IgE, respectively. The basic immunoglobulin (antibody) structural unit is generally a tetramer. Each tetramer is composed of two identical pairs of polypeptide chains, each pair having one “light” (about 25 kDa) and one “heavy” (about 50–70 kDa) chain. The N-terminus of each chain defines a variable какие симптомы при коронавирусе у взрослых какие симптомы при коронавирусе у взрослых region of about 100 to 110 or more amino acids primarily responsible for antigen recognition. The terms “variable light chain” (V L ) and “variable heavy chain” (V H ) refer, respectively, to these light and heavy chains.
