7B is an expanded view of the structural protein encoding region and predicted mRNA transcripts. Known structural protein encoding regions (dark grey почему вирус в китае назвали коронавирус boxes) and regions and reading frames for potential products X1–X5 (light gray boxes) are indicated. Lengths and map locations of the 3?-coterminal mRNAs expressed by the SARS-CoV are indicated, as predicted почему вирус в китае назвали коронавирус by identification of conserved transcriptional regulatory sequences. 7C is a digitized image of a nylon membrane почему вирус в китае назвали коронавирус showing Northern blot analysis of SARS-CoV mRNAs. Poly(A)+ RNA from infected Vero E6 cells was separated on a formaldehyde-agarose gel, transferred to a nylon membrane, and hybridized with a digoxigenin-labeled почему вирус в китае назвали коронавирус riboprobe overlapping the 3?-untranslated region.
Sizes of the SARS-CoV mRNAs were calculated by extrapolation from a log-linear fit of the molecular mass marker. Lane 1, SARS-CoV mRNA; lane 2, Vero E6 почему вирус в китае назвали коронавирус cell mRNA; lane 3, molecular mass marker, sizes in kB. SEQUENCE LISTING The nucleic and amino почему вирус в китае назвали коронавирус acid sequences listed in the accompanying sequence listing почему вирус в китае назвали коронавирус are shown using standard letter abbreviations for nucleotide bases, and three letter code for amino acids, почему вирус в китае назвали коронавирус as defined in 37 C.F.R.
Only one strand of each nucleic acid sequence is shown, but the complementary strand is understood as included by any reference to the displayed strand. In the accompanying sequence listing: SEQ ID NO: 1 shows the nucleic acid sequence of the SARS-CoV genome.
SEQ ID NO: 2 shows the amino acid sequence of the SARS-CoV polyprotein 1a (encoded by nucleic acid 265 to nucleic acid 13,398 of SEQ ID NO: 1). SEQ ID NO: 3 shows the amino acid sequence of the SARS-CoV polyprotein 1b (encoded by nucleic acid 13,398 to 21,482 of SEQ ID NO: 1). SEQ ID NO: 4 shows the amino acid sequence of the SARS-CoV почему вирус в китае назвали коронавирус S protein (encoded by nucleic acid 21,492 почему вирус в китае назвали коронавирус to 25,256 of SEQ ID NO: 1). SEQ ID NO: 5 shows the amino acid sequence of the SARS-CoV X1 protein (encoded by nucleic acid 25,268 to 26,089 of SEQ ID NO: 1). SEQ ID NO: 6 shows the amino acid sequence of the SARS-CoV X2 protein (encoded by nucleic acid 25,689 to 26,150 of SEQ ID NO: 1). SEQ почему вирус в китае назвали коронавирус ID NO: 7 shows the amino acid sequence of the SARS-CoV E protein (encoded by nucleic acid 26,117 to 26,344 of SEQ ID NO: 1). SEQ ID NO: 8 shows the amino acid sequence of the SARS-CoV M protein (encoded by nucleic acid 26,398 to 27,060 of SEQ ID NO: почему вирус в китае назвали коронавирус 1). SEQ ID NO: 9 shows the amino acid sequence of the SARS-CoV X3 protein (encoded by nucleic acid 27,074 to 27,262 of SEQ ID NO: 1).
SEQ ID NO: 10 shows the amino acid sequence of the SARS-CoV X4 protein (encoded by nucleic acid 27,273 to 27,638 of SEQ ID NO: 1).
SEQ ID NO: 11 shows the amino почему вирус в китае назвали коронавирус acid sequence of the SARS-CoV X5 protein (encoded by nucleic acid 27,864 to 28,115 почему вирус в китае назвали коронавирус of SEQ ID NO: 1). SEQ ID NO: 12 shows the amino acid sequence of the SARS-CoV N protein (encoded by nucleic acid 28,почему вирус в китае назвали коронавирус 120 to 29,385 of SEQ ID NO: 1).
SEQ ID NOs: 13–15 show the nucleic acid sequence of several SARS-CoV-specific oligonucleotide primers. SEQ ID NOs: 16–33 show the nucleic acid sequence of several oligonucleotide primers/probes used for real-time reverse почему вирус в китае назвали коронавирус transcription-polymerase chain reaction (RT-PCR) SARS-CoV assays. SEQ ID NOs: 34–35 show the nucleic acid sequence of two degenerate primers designed to anneal to sites почему вирус в китае назвали коронавирус encoding conserved coronavirus amino acid motifs.
SEQ ID NOs: 36–38 show the nucleic acid sequence of several oligonucleotide primers/probe used as controls in почему вирус в китае назвали коронавирус real-time RT-PCR assays. Abbreviations M: coronavirus membrane protein N: coronavirus nucleoprotein ORF: open reading frame PCR polymerase chain reaction RACE: 5? rapid amplification of cDNA ends RT-PCR: reverse transcription-polymerase chain reaction S: почему вирус в китае назвали коронавирус coronavirus spike protein SARS: severe acute respiratory syndrome SARS-CoV: severe acute respiratory syndrome-associated coronavirus TRS: transcriptional regulatory sequence II. Terms Unless otherwise noted, technical terms are used according to conventional usage. Definitions почему вирус в китае назвали коронавирус of common terms in molecular biology may be found in Benjamin Lewin, Genes VII , published by Oxford University Press, 2000 (ISBN 019879276X); Kendrew et al. (eds.), The Encyclopedia of Molecular Biology , published by Blackwell Publishers, 1994 (ISBN 0632021829); and Robert A. Meyers (ed.), Molecular Biology and Biotechnology: a Comprehensive Desk Reference , published by Wiley, John & Sons, Inc., 1995 (ISBN 0471186341); and other similar references. As used herein, the singular terms “a,” “an,” and “the” include plural referents unless context clearly indicates otherwise.
Similarly, the word “or” is intended to include “and” unless the context clearly indicates otherwise. Also, as used herein, the term “comprises” means “includes.” Hence “comprising A or B” почему вирус в китае назвали коронавирус means including A, B, or A and B.
